Lesson Summary

This lesson reviews quality control procedures and troubleshooting.

Learning Objectives

• Perform routine quality control.
• Identify staining problems.
• Implement corrective actions.

Content

Positive Control

Recommended controls:

• Liver
• Kidney
• Small intestine

Common Problems

Weak Staining

Possible Causes:

• Expired Schiff reagent
• Inadequate oxidation
• Poor fixation

Excessive Background

Possible Causes:

• Overstaining
• Inadequate washing
• Contaminated reagents

No PAS Reaction

Possible Causes:

• Failed Schiff reagent
• Incorrect reagent preparation

Key Takeaway

Daily quality control is essential for reliable PAS staining.